WebCisplatin is nephrotoxic. The mechanism underlying this organ-specific toxicity is unknown. We hypothesize that cisplatin is metabolized via a γ -glutamyl transpeptidase (GGT) and cysteine S -conjugate β -lyase-dependent pathway that has been shown to activate several haloalkenes to nephrotoxins. WebCisplatin (24 mg/kg body weight) was given by a single intraperitoneal injection. Mice were killed at day 1 or day 3 after cisplatin administration, blood and kidney samples were harvested for further analysis. Renal ischemia-reperfusion was induced in mice as described previously [ 21, 22 ].
High pressure liquid chromatography and mass spectrometry ...
WebThis enzyme uses cysteine conjugates as substrates, releasing the thiol derivative of the original xenobiotic, ammonia, and pyruvic acid, the thiol derivative then undergoing S-methylation to give rise to the methylthio derivative. WebThis hypothesis was tested by pre-treating C57BL/6j mice with suramin prior to cisplatin. Our data indicates that suramin protects the kidney from injury by decreasing cisplatin-induced decreases in kidney function. Renal histology indicated that suramin significantly protected from cisplatin-induced AKI. Data indicate that suramin side effects of andarine
Genetic Polymorphisms and Cisplatin- Related Nephrotoxicity
WebAug 28, 2009 · Purpose The mechanisms for cisplatin-induced renal cell injury have been the focus of intense investigation for many years with a view to provide a more effective and convenient form of nephroprotection. BNP7787 (disodium 2,2′-dithio-bis ethane sulfonate; dimesna, Tavocept™), is a water-soluble disulfide investigational new drug that is … WebThe first step is the conjugation to glutathione, followed by the cleavage by GGT to a cysteinyl-glycine conjugate, the cleavage by aminopeptidase to a cysteine S … WebNov 1, 2001 · The cysteine-conjugates are taken up into the cell and converted to a reactive thiol by either cysteine-conjugate β-lyase or S-oxidase. ... cisplatin-cysteinyl-glycine-conjugates, and cisplatin-cysteine-conjugates are all more toxic to proximal tubule cells than cisplatin. 25 We have also shown that inhibition of cysteine-conjugate β-lyase by ... side effects of androgel 1.62