Fosmid library production
WebEpicentre Copycontrol Fosmid Library Production Kit, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 86/100, based on 1 … WebJul 22, 2014 · Fosmid metagenomic libraries were constructed with the use of the CopyControl Fosmid Library Production Kit with pCC2FOS Vector (purchased from Epicentre Biotechnologies), according to manufacturer’s instructions.
Fosmid library production
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WebExcept genomic DNA, all the materials and protocol as mentioned above were supplied by the Copy Control TM Fosmid Library Production Kit (Epicentre, Madison, WI, United States). Two hundred clones were picked randomly and cultured overnight in 5 ml of LB liquid medium containing 12.5 μg/ml chloramphenicol and 50 μl of auto-induction solution ... WebMay 1, 2024 · A soil metagenomic fosmid library was constructed and the clones were screened for antimicrobial function. Our study has shown that heterologous production not only leads to enhanced yield but also provides a platform for further genetic manipulation to increase their efficacy. The peptides were having strong antimicrobial… Show more
Webhigh-quality fosmid DNA. The scalable protocol is based on a modified alkaline-lysis procedure that can be used with 1.5–100 mlof culture. Consistent yields of up to 0.6, 15 or 25 μg of fosmid DNA are obtained from 1.5, 40 or 100 ml cultures of a single-copy fosmid, respectively. Up to 4 µg of fosmid DNA can be isolated from 1.5 ml of WebDec 3, 2014 · The CopyControl™ Fosmid Library Production Kits* provide an efficient and improved method for constructing a library of approximately 40 kb clones. The …
http://www.nwbiotec.com/index.php?g=asset&m=download&a=dw&smurl=5044 WebProduction of a beta-carotene through expression of biosynthetic pathway in S. cerevisiae with the carbon source of a xylose: ... Construction of a library with the Golden Gate Assembly method for violacein production in Y. lipolytica: 70.04 mg/L ... Construction of metagenomic fosmid library: Amlakhadi metagenomic library: HPPD: E. coli (Amin ...
WebCreative Biogene offers fosmid library construction services for your scientific research as follows: DNA extraction and shearing. Vector construction and infection. Production of a …
WebJan 1, 2010 · Here, we describe one protocol for the construction of small-insert libraries and one for large-insert fosmid libraries. Both methods have been proven to be suitable for cloning of DNA purified from various environmental samples, including soil, ice, and compost ( 6 – 8 ). 2 Materials 2.1 Metagenomic DNA ipad air cases for boysWebMay 11, 2012 · High molecular weight genomic DNA was used to clone the P-SSP7 genome into the pCC1FOS fosmid using the CopyControl Fosmid Library Production kit (Epicentre Biotechnologies) following the manufacturer’s instructions. Briefly, the phage DNA was treated with T4 DNA polymerase and T4 polynucleotide kinase to generate … ipad air case fit new ipadWebMar 9, 2024 · Sequencing was performed using primers indicated in the CopyControl library production kit for pCC2FOS vector (FWD: 5′‐GTACAACGACACCTAGAC‐`3 ... against the insert‐fosmid end sequences—TAGS of each of the 40 selected fosmids, resulted in 18 contigs successfully tagged to their corresponding fosmid in both ends (5′ and 3′ ends). ... ipad air case ruggedWebSep 23, 2009 · The whole process of fosmid library production and automated colony picking takes at least 7-10 days as there are many incubation steps included. However, … open kitchen shelf decorWebA Fosmid Library is a type of genetic engineering tool used to clone large fragments of DNA. By isolating fragments of DNA, researchers can study the function of genes and their roles in evolution. The library is made up of circular DNA molecules called Fosmids, which contain approximately 40-50 kilobases of DNA, allowing for the study of ... ipad air case nzWebThe primary acquired skills in the fields of Molecular Biology and Microbiology. My research interests span the areas of PCR¬ based gene synthesis, bacterial transformation, and culture, Fosmid ... open kitchen shelves cabinetsWebLucigen also offers the CopyControl Fosmid Library Production Kit. This kit utilizes a strategy of cloning blunt-ended DNA fragments, generated by random shearing of DNA, to produce complete and unbiased genomic libraries. Greater than 106 clones are produced in a single experiment. open kitchen shelves finish