Phosphospecific antibody
WebPhospho-Specific Antibodies Bio-Rad Phospho-Specific Antibodies For analyzing cell signaling pathway activation Our phospho-specific antibody range References For … WebThis antibody is predicted to react with bovine, canine, human, mouse and non-human primate based on 100% sequence homology. In Western blot, this antibody is specific for ∽78 kDa synapsin I doublet phosphorylated at Ser549. Immunolabeling can be blocked by lambda-phosphatase treatment.
Phosphospecific antibody
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WebJan 1, 2000 · Phosphospecific Antibodies Reveal Focal Adhesion Kinase Activation Loop Phosphorylation in Nascent and Mature Focal Adhesions and Requirement for the Autophosphorylation Site1 Cell Growth & Differentiation American Association for Cancer Research Articles January 01 2000 WebAug 1, 2002 · Phosphospecific acetyl-CoA carboxylase (ACC) antibody was raised against the peptide TMRPSMSpGLHLVK (residues 221–233 of human ACC2; Sp = phosphoserine) as described for anti-pT172 ( 24 ). This antibody appears to recognize the homologous AMPK sites on ACC1 (Ser-79) and ACC2 (Ser-221).
WebThese phospho-specific antibodies can thus be used to track the activity of a protein, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing the ... WebMost phosphatases only dephosphorylate serine and threonine amino acids. If you are testing the phosphospecificity of a tyrosine phosphospecific antibody, lambda phosphatase is essential. The second dephosphorylation treatment is done with an alkaline phosphatase, a significantly more potent enzyme than lambda phosphatase.
WebPhospho-specific antibodies. Antibodies that discriminate between the phosphorylated and the non-phosphorylated state of a protein, is one of the most frequently asked for types of PTM specific antibodies. These could be made as monoclonal antibodies but are more often produced as antigen specific affinity purified polyclonal antibodies. WebAntibodies, Phospho-Specific Antibodies directed against immunogen-coupled phosphorylated PEPTIDES corresponding to amino acids surrounding the …
WebAntiphosphotyrosine antibodies have been proven very successful in purification, but fewer reports have been published using antibodies against phosphoserine- or phosphothreonine-containing proteins. IMAC enrichment is based on phosphate affinity for immobilized metal chelated to the resin.
WebImmunogen: This antibody was produced against a chemically synthesized phosphopeptide derived from human p53, corresponding to the serine 6 phosphorylation site. Specificity: This antibody recognizes p53 when phosphorylated at serine 6. p53, a critically important mammalian cell-cycle checkpoint protein, arrests the cell cycle and in many flint arrowhead cookware historyWebPhospho Purification Strategy: To isolate antibodies specific to the phospho epitope, serum will first be affinity purified against the non-phosphorylated peptide prior to being run through the phosphorylated peptide column. Comprehensive ELISA testing against both peptides confirms the specificity of the purified antibodies. greater lafayette commerce facebookgreater lafayette commerce golf outingWebMonoclonal Antibody for studying Thr-P. Cited in 2 publications. Highly specific and rigorously validated in-house, Phospho-Threonine (42H4) Mouse Monoclonal Antibody (CST #5005) is ready to ship. greater lafayette commerce indianaWebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse Monoclonal Antibody (CST #5282) is ready to ship. flint arrowhead cookwareWebPhospho-specific antibodies allow for the phosphorylation state of individual protein residues to be determined by standard, immunoblotting techniques. 26 Several phospho … flint arrow headWebPhospho-specific antibodies, which can discriminate between the phosphorylated and non-phosphorylated forms, have emerged as demonstrated assets in the study of signal … flint arrowhead images