WebJun 15, 2024 · The levels of transcription for IL-5 and IL-13 in individual Tc2 cells in whole blood or Tc2 cultures were analysed with a PrimeFlow RNA Assay kit (eBioscience) according to the manufacturer’s ... WebMar 31, 2024 · For sgRNA and activator screening, TaqMan fast advanced cells-to-CT kit (Thermo Fisher Scientific) was utilized with a 96-well format. Two methods were employed ... a PrimeFlow assay (Invitrogen, 88-18005-204) was performed according to manufacturer’s instructions. Probes against mouse B4galnt2 mRNA (Invitrogen, VB1 …
Improved detection of bovine viral diarrhea virus in bovine …
WebInvitrogen™PrimeFlow™ RNA Assay Kit, 40 tests . This PrimeFlow™ RNA Assay is an in situ hybridization assay that combines the power of branched-DNA technology with the single-cell resolution of flow cytometry, enabling the simultaneous detection of up to four RNA targets in combination with immunophenotyping for cell surface and intracellular … WebAn in situ hybridization assay that combines the power of branched-DNA technology with the single-cell resolution of flow cytometry, enabling the detection of four RNA targets in … craigslist piano phoenix
PrimeFlow™ RNA Assay Kit
WebIn this kit, amylase activity is determined using a coupled enzymatic assay, which results in a colorimetric (405 nm) product, proportional to the amount of substrate, ethylidene-pNP-G7, cleaved by the amylase. One unit is the amount of amylase that cleaves ethylidene-pNP-G7 to generate 1.0 μmole of p -nitrophenol per minute at 25 °C. WebSep 1, 2024 · The novel assay described herein is a reliable and repeatable procedure that eliminates a need to optimize the assay for different samples. This PrimeFlow RNA Assay based technique provides the opportunity to stain cells for flow cytometry and microscopy and the capacity to evaluate viral infection at the individual cell level, rather than ... WebPrimeFlow RNA Assay workflow summary In the PrimeFlow RNA Assay workflow, cells are first labeled with cell-surface antibodies, fixed and permeabilized, and then labeled with … craigslist phone number reply